F2RL1 Knockout cell line (Hep G2)

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F2RL1 Knockout cell line (Hep G2) Order

F2RL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3556
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
F2RL1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
F2RL1 Knockout cell line (Hep G2)
Species
Human
Gene Name
F2RL1
Gene ID
2150
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript F2RL1-201)
Validation: PCR+Sanger Sequencing
F2RL1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the F2RL1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, F2RL1 gene knockout F2RL1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
F2RL1 Knockout cell line (Hep G2)
F2RL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3556
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • F2RL1 Knockout cell line(293T)

    F2RL1 Knockout cell line(293T)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-H561
    YKO-H561(293T)
  • F2RL1 Knockout cell line (HeLa)

    F2RL1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25036
    YKO-XN25036(HeLa)
  • F2RL1 Knockout cell line (HCT 116)

    F2RL1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25035
    YKO-XN25035(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • F2RL1 Knockout cell line(293T)

    F2RL1 Knockout cell line(293T)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-H561
      inquiry   More details
  • F2RL1 Knockout cell line (HeLa)

    F2RL1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25036
      inquiry   More details
  • F2RL1 Knockout cell line (HCT 116)

    F2RL1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25035
      inquiry   More details

F2RL1 Knockout cell line (Hep G2) Order

F2RL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3556
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
F2RL1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
F2RL1 Knockout cell line (Hep G2)
Species
Human
Gene Name
F2RL1
Gene ID
2150
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript F2RL1-201)
Validation: PCR+Sanger Sequencing
F2RL1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the F2RL1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, F2RL1 gene knockout F2RL1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
F2RL1 Knockout cell line (Hep G2)
F2RL1 Knockout cell line (Hep G2)
Catalog#
YKO-HT3556
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • F2RL1 Knockout cell line(293T)

    F2RL1 Knockout cell line(293T)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-H561
    YKO-H561(293T)
  • F2RL1 Knockout cell line (HeLa)

    F2RL1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25036
    YKO-XN25036(HeLa)
  • F2RL1 Knockout cell line (HCT 116)

    F2RL1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25035
    YKO-XN25035(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • F2RL1 Knockout cell line(293T)

    F2RL1 Knockout cell line(293T)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-H561
      inquiry   More details
  • F2RL1 Knockout cell line (HeLa)

    F2RL1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25036
      inquiry   More details
  • F2RL1 Knockout cell line (HCT 116)

    F2RL1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: F2RL1
    Gene ID: 2150
    Size: 1*10^6
    Catalog#: YKO-XN25035
      inquiry   More details

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